How do you make edta

Concentration ml ml ml ml mM 3. Save my name, email, and website in this browser for the next time I comment. Preparation of EDTA solution. Table of Contents. Add a header to begin generating the table of contents. Ca EDTA. A strong base must be used in conjunction with water to create the solution. Use your balance to measure Begin stirring the solution with the magnetic stirrer when you add the EDTA.

Use your balance to measure 20 g of sodium hydroxide NaOH and add roughly half of it to the solution. Continue stirring. It will start to dissolve into solution as the pH approaches 8. Once it has dissolved completely, add one more gram of NaOH to the solution and stop the stirrer. The directions contained here will make a 0. Add a magnetic flea and place on a magnetic stirring plate to mix the solution.

Add a pH meter into the solution to observe the pH. To dissolve the salt, add sodium hydroxide NaOH pellets to the solution. Add a few pellets at a time and wait until the pellets have fully dissolved before adding more. It may take around 2 g of NaOH pellets before the pH is at 8. Once fully dissolved this will take some time so be patient , top up the solution to mL using distilled water, if necessary. To sterilise, autoclave the solution on a liquid cycle 20 min at 15 psi.

Helmenstine holds a Ph. She has taught science courses at the high school, college, and graduate levels. Facebook Facebook Twitter Twitter. Updated September 16, Featured Video. Cite this Article Format. Helmenstine, Anne Marie, Ph. How to Make a Phosphate Buffer Solution.